Enzymatic Activity and Content of Polycyclic Aromatic Hydrocarbons (PAHs) in Soils under Low-Stack Emission in Lublin

Barbara Futa, Elżbieta Jolanta Bielińska, Sławomir Ligęza, Szymon Chmielewski, Sylwia Wesołowska, Krzysztof Patkowski, Agnieszka Mocek-Płóciniak


Soils from the Lublin area exposed to low-stack emission from home heating and transport were investigated. Changes in soil enzymatic activity and polycyclic aromatic hydrocarbons (PAHs) content were analysed. Soil samples were collected in July and November 2016 and January 2017. Results of the study show that enzymatic activity and PAHs content depended on types of buildings, study period (intensification of pollutant emission from household heating), traffic volume and atmospheric air movement. Low enzymatic activities and high PAHs contents in the soils were observed in the autumn-winter period in the areas with dense single-family housing and located in the vicinity of streets with intense road traffic.


urban soils, enzymatic activity, low-stack emission, PAHs

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Adamczewska, M., Siepak, J., Gramowska, H., 2000. Studies of Levels of Polycyclic Aromatic Hydrocarbons in Soils Subjected to Anthropopressure in the City of Poznań. Polish Journal of Environmental Studies, 9: 305–321.

Baran, S., Oleszczuk, P., 2001. Determination of Polycyclic Aromatic Hydrocarbons in soils and Organic Waste by HPLC-UV (in Polish). Acta Agrophysica, 48: 7–16.

Baran, S., Bielińska, E.J., Oleszczuk, P., 2004. Enzymatic Activity in an Airfild Soil Polluted with Polycyclic Aromatic Hydrocarbons. Geoderma, 118: 221–232.

Bielińska, E.J., Ligęza, S., Chudecka, J., Tomaszewicz, T., 2009. Soil Transformations in an Urban Landscape. In: Bieniek, B. (ed.), Soil of Chosen Landscapes, ISBN 978-83-929462-4-3, Univ. of Warmia and Mazury in Olsztyn, pp. 85–98.

Bielińska, E.J., Kawecka-Radomska, M., Kłos, A., 2011. Impact of Urban Factors on the Content of Polycyclic Aromatic Hydrocarbons in Soils of Park Gardens (in Polish). Soil Science Annual, 62, 2: 16–22.

Bielińska, E.J., Futa, B., Mocek-Płóciniak, A., 2014a. Soils enzymes as bio-indicators of soil health and quality (in Polish). Towarzystwo Wydawnictw Naukowych “LIBROPOLIS”, Lublin.

Bielińska, E.J., Futa, B., Mocek-Płóciniak, A., Kawecka-Radomska, M., 2014. The Relationship Between the Activity of Dehydrogenases and Phosphatases and the Content of Polycyclic Aromatic Hydrocarbons in Urban Soils. Journal of Research and Applications in Agricultural Engineering, 59, 3: 8–11.

Burton, D.L., Beauchamp, E.G., 1994. Profie Nitrous Oxide and Carbon Dioxide Concentration in a Soil Subject to Freezing. Soil Science Society of America Journal, 58: 115–122.

Caravaca, F., Rodán, A., 2003. Assessing Changes in Physical and Biological Properties in a Soil Contaminated by Oil Sludge under Semiarid Mediterranean Conditions. Geoderma 117: 53–61.

Carbrera, M.L., Kissel, D.L., Bock, B.R., 1994. Urea Hydrolysis in Soil. Effect of Urea Concentration and Soil pH. Soil Biology and Biochemistry, 23: 1121–1124.

Cousins, I.T., Kreibich, H., Hudson, L.E., Lead, W.A., Jones, K.C., 1997. PAHs in Soils: Contemporary UK Data and Evidence for Potential Contamination Problems Caused by Exposure of Samples to Laboratory Air. Science of the Total Environment, 203: 141–156.

Dębska, B., Banach-Szott, M., Rosa, E., Drąg, M., 2011. Effect of Soil Pollution with PAHs on Organic Matter Properties. Polish Journal of Soil Science, 44, 2: 97–103.

Díaz-Raviña, M., Acea, M.J., Carballas, T., 1995. Seasonal Changes in Microbial Biomass and Nutrient Flush in Forest Soil. Biology and Fertility of Soils, 19: 220–226.

Futa, B., Bielińska, E.J., Mocek-Płóciniak, A., 2016. The Use of Enzymatic Tests to Assess the Quality of Arable Soils Along Main Thoroughfares in Lublin. Journal of Research and Applications in Agricultural Engineering, 61, 3: 94–97.

Gschwend. P.M., Hites, R.A., 1981. Fluxes of Polycyclic Aromatic Hydrocarbons to Marine and Lacustrine Sediments in the Northeastern United States. Geochimica et Cosmochimica Acta, 45: 2359–2367.

Januszek, K., 1999. Aktywność enzymatyczna wybranych gleb leśnych Polski południowej w świetle badań polowych i laboratoryjnych. Zeszyty Naukowe AR w Krakowie, Rozprawy, 250.

Junter, G.A., Coquet, L., Vilain, S., Jouenne, T., 2002. Immobilized-Cell Physiology, Current Data and Potentialities of Proteomics. Enzyme and Microbial Technology, 31: 201–212.

Kanaly, R.A., Harayama, S., 2000. Biodegradation of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Bacteria. Journal of Bacteriology, 182: 2059–2067.

Kluska, M., Kroszczyński, W., 2000. The Content of Polycyclic Aromatic Hydrocarbons Nearby Roads with Intensive Car Traffi (in Polish). Chemia i Inżynieria Ekologiczna, 7: 263–273.

Li, T., Meng, L., Herman, U., Lu, Z., Crittenden, J., 2015. A Survey of Soil Enzyme Activities along Major Roads in Beijing: The Implications for Traffi Corridor Green Space Management. International Journal of Environmental Research and Public Health ISSN 1660-4601, 12: 12475–12488, DOI: 10.3390/ijerph121012475

Lipińska, A., Kucharski, J., Wyszkowska, J., 2014. The Effect of Polycyclic Aromatic Hydrocarbons on the Structure of Organotrophic Bacteria and Dehydrogenase Activity in Soil. Polycyclic Aromatic Compounds, 34(1): 35–53, DOI: 10.1080/10406638.2013.844175

Maliszewska-Kordybach, B. 1996. Polycyclic Aromatic Hydrocarbons in Agricultural Soils in Poland – Preliminary Proposals for the Criteria for the Evaluation of the Level of Soils Pollution. Applied Geochemistry, 11: 121–127.

Maliszewska-Kordybach, B., Smreczak, B., 1997. The Content of Polycyclic Aromatic Hydrocarbons (PAH) in Agricultural Soils in Lublin District (in Polish). Soil Science Annual, 1–2: 95–110.

Oleszczuk, P., Baran, S., 2005. The Content of Polycyclic Aromatic Hydrocarbons in Soils under Different Anthropogenic Inflence (in Polish). Soil Science Annual, 56, 3/4: 67–77.

Russel, S., Wyczółkowski, A.I. (eds.), 2005. Methods for Determining Enzymes in Soil (in Polish). Acta Agropphysica. Rozprawy i Monografi, 3: 63–74.

Sims, R.C., Overcash, M.R., 1983. Fate of Polynuclear Aromatic Compounds (PNAs) in Soil-Plant Systems. In: Gunther, F.A. (eds.), Residue Reviews, 88: 1–66.

Siwik-Ziomek, A., Lemanowicz, J., 2016. The Inflence of Fertilization with Phosphorus, Sulphate, Carbon and Nitrogen Content on Hydrolases Activities in Soil. Polish Journal of Soil Science, 49, 1: 49–60, DOI: 10.17951/pjss/2016.49.1.49

Stępniewska, Z., Samborska, A., 2002. Dynamics of Changes in Urease Activity in Fields Sown with a Mixture of Grasses: Alopecurus Pratensis, Phalaris Arundinacea, Festula Pratensis Irrigated With Urban Sewage (in Polish). III Ogólnopolskie Sympozjum Naukowo-Technicznego „Bioremediacja gruntów”, 89–96, Wisła-Jarzębata 2002.

Świetlik, R., Kowalczyk, D., Dojlido, R., 1998. Soil Contamination by PAHs in the Vicinity of a Heat-Generating Plant (in Polish). Ochrona Środowiska, 69: 21–24.

Tabatabai, M.A., Bremner, J.M., 1969. Use of p-nitrophenyl Phosphate for Assay of Soil Phosphatase Activity. Soil Biology and Biochemistry, 1: 301–307.

Thalmann, A., 1968. Zur Methodik der Bestimmung der Dehydrogenase Aktivität in Boden mittels Triphenyltetrazoliumchlorid (TTC). Landwirtschaftliche Forschung, 21: 249–258.

Wild, S.R., Jones, K.C., 1995. Polynuclear Aromatic Hydrocarbons in the United Kingdom Environment: A Preliminary Source Inventory and Budget. Environmental Pollution, 88: 91–108.

Wyszkowska, J., Kucharski, M., Kucharski, J., 2006. Application of the Activity of Soil Enzymes in the Evaluation of Soil Contamination with Diesel Oil. Polish Journal of Environmental Studies, 15, 2a: 212–221.

Zantua, M.I., Bremner, J.M., 1975. Comparison of Methods of Assaying Urease Activity in Soils. Soil Biology and Biochemistry, 7: 291–295.

Zerbe, J., Sobczyński, T., Siepak, J., 1995. Soil Pollution in Plots with Heavy Metals and Polycyclic Aromatic Hydrocarbons (in Polish). Przyroda i Człowiek, 5: 133–141.

DOI: http://dx.doi.org/10.17951/pjss.2017.50.1.63
Data publikacji: 2017-10-03 12:49:19
Data złożenia artykułu: 2017-05-22 23:27:02


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