Cytogenetics of Tradescantia spathacea (syn. Rhoeo spathacea). A review

Hieronim Golczyk


As a favorable PTH (permanent translocation heterozygosity) model, Tradescantia spathacea (synon. Rhoeo spathacea) offers an unusual opportunity to address crucial cytogenetic problems, among them complex chromosome rearrangements, recognition and segregation of translocated genome parts, the relation of crossing-over to synaptic processes. Here I present the existing knowledge on T. spathacea with the intention to reestablish it for advanced cytogenetic research.


chromosomes; karyotype evolution; meiosis; meiotic ring; Rhoeo; permanent translocation heterozygosity (PTH); rDNA; Tradescantia spathacea; translocations

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Akemine T. 1937. Non-disjunction of the meiotic chromosomes in Rhoeo discolor Hance. Jap. J. Genet. 13, 31–36.

Baker R. F., Mertens T. R. 1975. Meiosis in variegated and anthocyjaninless varieties of Rhoeo. J. Hered. 66, 381–383.

Barzel A., Kupiec M. 2008. Finding a match: how do homologous sequences get together for recombination? Nat. Rev. Genet. 9, 27–37.

Belling J. 1927. The attachments of chromosomes at the reduction division in flowering plants. J. Genet. 18, 177–205.

Bhaduri P. N. 1942. Application of new technique to cytogenetical reinvestigation of the genus Tradescantia. J. Genet. 44, 87–127.

Britton-Davidian J., Cazaux B., Catalan J. 2012. Chromosomal dynamics of nucleolar organizer regions (NORs) in the house mouse: micro-evolutionary insights. Heredity 108, 68–74.

Brown S. P., Levin D. A. 2011. Social dilemmas among supergenes: intragenomic sexual conflict and a selfing solution in Oenothera. Evolution 65, 3360–3367.

Burnham C. R. 1956. Chromosomal interchanges in plants. Bot. Rev. 22, 419–552.

Burns J. H., Faden R. B., Steppan S. J. 2011. Phylogenetic studies in the Commelinaceae subfamily Commelinoideae inferred from nuclear ribosomal and chloroplast DNA sequences. Syst. Bot. 36, 268–276.

Carniel K. 1960. Beitrage zum Sterilitäts- and Befruchtungsproblem von Rhoeo discolor. Chromosoma

, 456–462.

Church K., Moens P. B. 1976. Centromere behaviour during interphase and meiotic prophase in Allium fistulosum from 3-D E.M. reconstruction. Chromosoma 56, 249–263.

Cleland R. E. 1972. Oenothera. Cytogenetics and Evolution. Academic Press, London and New York.

Coleman L. C. 1941. The relation of chromocenters to the differential segments in Rhoeo discolor Hance. Am. J. Bot. 28, 742–748.

Cuadrado A., Golczyk H., Jouve N. 2009. A novel, simple and rapid nondenaturing FISH (ND-FISH) technique for the detection of plant telomeres. Potential used and possible target structures detected. Chromosome Res. 17, 755–762.

Darlington C. D. 1929. Chromosome behaviour and structural hybridity in the Tradescantiae. J. Genet. 21, 207–286.

Darlington C. D. 1931. The cytological theory of inheritance in Oenothera. J. Genet. 24, 405–474.

Darlington C. D. 1937. Chromosome behaviour and structural hybridity in the Tradescantiae. II. J. Genet. 35, 259–280.

Desai S. 1965. A cytological study of a triploid Rhoeo discolor. Cytologia 30, 260–265.

Dietrich W., Wagner W. L., Raven P. H. 1997. Systematics of Oenothera section Oenothera subsection Oenothera (Onagraceae). Sys. Bot. Monogr. 50. The American Society of Plant Taxonomists.

Emerson S. 1935. The genetic nature of De Vries’s mutations in Oenothera lamarckiana. Am. Nat. 69, 545–559.

Faden R. B. 1998. Commelinaceae. In: The Families and Genera of Vascular Plants 4, Ed. Kubitzki K., Berlin, Springer, pp. 109–127.

Ferdous M., Higgins J. D., Osman K., Lambing C., Roitinger E., Mechtler K., Armstrong S. J., Perry R., Pradillo M., Cuñado N., Franklin F. C. 2012. Inter-homolog crossing-over and synapsis in Arabidopsis meiosis are dependent on the chromosome axis protein AtASY3. PLoS Genet. 8(2), e1002507.

Flagg R. O. 1958. A mutation and an inversion in Rhoeo discolor. J. Hered. 49, 185–188.

Garcia-Velazquez A. 1991. Cytogenetical studies in Rhoeo spathacea (Commelinaceae). I. A desynaptic and second division restitution mutant. Genome 34, 895–899.

Garcia-Velazquez A. 1994. A desynaptic mutant in Rhoeo spathacea (Commelinaceae). Caryologia 59, 399–404.

Gernand D., Golczyk H., Rutten T., Ilnicki T., Houben A., Joachimiak A. J. 2007. Tissue culture triggers chromosome alterations, amplification and transposition of repeat sequences in Allium fistulosum. Genome 50, 435–442.

Gladstone M. N., Obeso D., Chuong H., Dawson D. S. 2009. The synaptonemal complex protein Zip1 promotes bi-orientation of centromeres at meiosis I. PLoS Genet. 5, e1000771.

Golczyk H. 2011. Cytogenetics of the permanent translocation heterozygote Rhoeo spathacea var. variegata. Implications for complex chromosome rearrangements in Rhoeo. Caryologia 64, 325–334.

Golczyk H. 2011. Breakdown of the balanced lethals in Rhoeo. The structure of the alethal Renner complex of the homozygotic stock of Rhoeo. Cytogenet. Genome Res. 134, 229–233.

Golczyk H. 2011. The arrangement of pericentromeres during meiotic prophase I in the permanent

translocation heterozygote Rhoeo spathacea. Caryologia 64, 197–202.

Golczyk H. 2011. Structural heterozygosity, duplication of telomeric (TTTAGGG)n clusters and B chromosome architecture in Tradescantia virginiana L. Cytogenet. Genome Res. 134, 234–242.

Golczyk H., Joachimiak A. 2003. NORs in Rhoeo revisited. Caryologia 56, 31–35.

Golczyk H., Hasterok R., Joachimiak A. J. 2005. FISH-aimed karyotyping and characterization of Renner complexes in permanent heterozygote Rhoeo spathacea . Genome 48, 145–153.

Golczyk H., Musiał K., Rauwolf U., Meurer J., Herrmann R. G., Greiner S. 2008. Meiotic events in Oenothera – a non-standard pattern of chromosome behaviour. Genome 51, 952–958.

Golczyk H., Joachimiak A., Hasterok R. 2008. Pericentromeric GC-rich chromatin in Rhoeo. Evidence from soma and germ-line. Caryologia 61, 388–391.

Golczyk H., Hasterok R., Szklarczyk M. 2010. Ribosomal DNA, tri- and bipartite pericentromeres in the permanent translocation heterozygote Rhoeo spathacea. Cell. Mol. Biol. Lett. 15, 65–664.

Goldblatt P. 1980. Uneven diploid chromosome numbers and complex heterozygosity in Homeria (Iridaceae). Sys. Bot. 5, 337–340.

Grant V. 1975. Genetics of Flowering Plants. Columbia University Press. New York and London.

Grützner F., Ashley T., Rowell D. M., Marshall Graves J. A. 2006. How did the platypus get its sex chromosome chain? A comparison of meiotic multiples and sex chromosomes in plants and animals. Chromosoma 115, 75–88.

Harte C. 1994. Oenothera. Contributions of a plant to biology In: Frankel R, Grossman M, Linskens HF, Maliga P and Riley R (eds.). Monographs on Theoretical and Applied Genetics. Verlag. Berlin, Heidelberg, New York.

Haselwarter A. 1937. Untersuchungen zur Physiologie der Meiosis V. Z. Bot. 31, 273–328.

Hernández-Hernández A, Vázquez-Nin G. H., Echeverría O. M., Recillas-Targa F. 2009. Chromatin structure contribution to the synaptonemal complex formation. Cell. Mol. Life. Sci. 66, 1198–208.

Holsinger K. E., Ellstrand N. C. 1984. The evolution and ecology of permanent translocation heterozygotes. Am. Nat. 124, 48–71.

Higgins J. D., Perry R. M., Barakate A., Ramsay L., Waugh R., Halpin C., Armstrong S. J, Franklin F. C. 2012. Spatiotemporal asymmetry of the meiotic program underlies the predominantly

distal distribution of meiotic crossovers in barley. Plant Cell 24, 4096–40109.

Huang M., Li H., Zhang L., Gao F., Wang P., Hu Y., Yan S., Zhao L., Zhang Q., Tan J., Liu X., He S., Li L. 2012. Plant 45S rDNA clusters are fragile sites and their instability is associated with epigenetic alterations. PLoS One. 7(4), e35139.

Hunt D. R. 1986. Campelia, Rhoeo, Zebrina united with Tradescantia. Kew Bull. 41, 401–405.

Inagaki A., Schoenmakers S., Baarends W. M. 2010. DNA double strand break repair, chromosome

synapsis and transcriptional silencing in meiosis. Epigenetics 5, 255–266.

Jahan B., Vahidy A. A. 1994. Meiotic chromosome configurations in Rhoeo spathacea (Swartz) Stearn. Pak. J. Bot. 26, 63–67.

James S. H. 1965. Complex hybridity in Isotoma petraea. I. The ocurrence of interchange heterozygosity, autogamy and balanced lethal system. Heredity 20, 341–353.

Jin Q., Trelles-Sticken E., Scherthan H., Loidl J. 1998. Yeast nuclei display prominent centromere clustering that is reduced in nondividing cells and meiotic prophace. J. Cell Biol. 141, 21–29.

Johnson M. T. J. 2011. The contribution of evening primrose (Oenothera biennis) to a modern synthesis of evolutionary ecology. Popul. Ecol. 53, 9–21.

Johnson M. T. J., FitzJohn R. G., Smith S. D., Rausher M., D., Otto S. P. 2011. Loss of sexual recombination and segregation is associated with increased diversification in evening primroses. Evolution 65, 3230–3240.

Kato K. 1930. Cytological studies of pollen mother cells of Rhoeo discolor Hance, with special reference to the question of the mode of syndesis. Mem. Coll. Sci. Kyoto Imp. Univ. Ser. B. 5, 139–161.

Kenton A., Davies A., Jones K. 1987. Identification of Renner complexes and duplications in permanent hybrids of Gibasis pulchella (Commelinaceae). Chromosoma 95, 424–434.

Kirkpatrick M. 2010. How and why chromosome inversions evolve. PLoS Biol. 8, e1000501.

Koller P. 1932. Further studies in Tradescantia virginiana var. humilis and Rhoeo discolor.

J. Genet. 26, 81–96.

Kumar K. K, Nagpal R., Arun A. 2011. Spectrum of chromosomal configurations in pollen mother cells of Rhoeo spathacea (Swartz) Stearn. Caryologia 64, 135–146.

Langeland K. A., Craddock Burks K (Eds.). 1998. Identification and biology of non-native plants in Florida’s natural areas. University of Florida, Gainesville, pp: 24–25.

Levin D. A. 2002. The Role of Chromosomal Change in Plant Evolution. Oxford series in Ecology and Evolution. Oxford University Press, New York.

Lin Y. J. 1979a. Chromosome distribution and catenation in Rhoeo spathacea var. concolor. Chromosoma 71, 109–127.

Lin Y. J. 1979b. Fine structure of meiotic prophase chromosomes amd modified synaptonemal complexes in diploid and triploid Rhoeo spathacea. Chromosoma J. Cell Sci. 37, 69–84.

Lin Y. J. 1980. Chromosome behaviour of Rhoeo spathacea var. variegata. Cytobios 27, 113–128.

Lin Y. J. 1982. Temperature and chiasma formation in Rhoeo spathacea var. variegata. Genetica 60, 25–30.

Lin Y. J., Paddock E. F. 1973a. Ring-position and frequency of adjacent distribution of meiotic chromosomes in Rhoeo spathacea. Am. J. Bot. 60, 685–690.

Lin Y. J., Paddock E. F. 1973b. Ring-position and frequency of chiasma failure in Rhoeo spathacea. Am. J. Bot. 60, 1023–1027.

Lin Y. J., Paddock E. F. 1978. Identification of complexes in a spntaneous triploid Rhoeo spathacea. Chromosoma 67, 97–108.

Maguire M. P. 1995. Is the synaptonemal complex a disjunction machine? J. Hered. 86, 330–340.

Mertens T. 1973. Meiotic chromosome behaviour in Rhoeo spathacea. J. Hered. 64, 365–368.

McKim K. S., Green-Marroquin B. L., Sekelsky J. J., Chin G., Steinberg C., Khodoson R., Hawley R. S. 1998. Meiotic synapsis in the absence of recombination. Science 279, 876–878.

McQuade H. A., Wells B. 1975. The synaptinemal complex in Rhoeo spathacea. J. Cell Sci. 17, 349–369.

Moens P. B. 1972. Fine structure of chromosome coiling at meiotic prophase in Rhoeo discolor. Can. J. Genet. Cytol. 14, 801–808.

Natarajan A.T., Natarajan S. 1972. The heterochromatin of Rhoeo discolor. Hereditas 72, 323–330.

Östergren G. 1951. The mechanism of co-orientation in bivalents and multivalents. The theory of orientation by pulling. Hereditas 37, 85–156.

Pettenati M. J. 1987. Giemsa C-banding of Rhoeo (Commelinaceae). Genetica 74, 219–224.

Poddar S., Datta A. K., Dana S. 1998. Cytogenetics of desynaptic mutants in homozygote stock of Rhoeo spathacea var. concolor. The Nucleus 41, 115-119.

Rauwolf U., Golczyk H., Meurer J., Herrmann R. G., Greiner S. 2008. Molecular marker systems

for Oenothera genetics. Genetics 180, 1289–1306.

Rauwolf U., Greiner S, Mráček J., Rauwolf M., Golczyk H., Mohler V., Herrmann R. G., Meurer J. 2011. Uncoupling of sexual reproduction from homologous recombination in homozygous Oenothera species. Heredity, 107: 87–94.

Rickards G. K. 1983. Orientation behavior of chromosome multiples of interchange (reciprocal translocation) heterozygotes. Ann. Rev. Genet. 17, 443–498.

Rosales-Reyes T., de la Garza M., Arias-Castro C., Rodríguez-Mendiola M., Fattel-Fazenda S., Arce-Popoca E., Hernández-García S., Villa-Treviño S. 2008. Aqueous crude extract of Rhoeo discolor, a Mexican medicinal plant, decreases the formation of liver preneoplastic foci in rats. J. Ethnopharmacol. 115, 381–386.

Satterfield S. K., Mertens T. R. 1972. Rhoeo spathacea: A tool for teaching meiosis and mitosis.

J. Hered. 63, 375–378.

Sax K. 1931. Chromosome ring formation in Rhoeo discolor. Cytologia 3, 36–53.

Sax K, Anderson E. 1933. Segmental interchange in chromosomes of Tradescantia. Genetics 18, 53–67.

Schubert I., Wobus U. 1985. In situ hybridization confirms jumping nucleolus organizing regions in Allium. Chromosoma 92, 143–148.

Stack S. M., Soulliere D. L. 1984. The relation between synapsis and chiasma formation in Rhoeo spathacea. Chromosoma 90, 72-83.

Stearn W. T. 1957. The boat-lily (Rhoeo spathacea). Baileya 5, 195–198.

Stebbins G. L. 1950. Variation and evolution in plants. Columbia University Press, New York.

Suzuki T., Ide N., Tanaka I. 1997. Immunocytochemical visualization of the centromeres during male and female meiosis in Lilium longiflorum. Chromosoma 106, 435–445.

Swanson C. P. 1940. The distribution of inversions in Tradescantia. Genetics 25, 438–465.

Takeo S., Hawley R. S. 2012. Rumors of its disassembly have been greatly exaggerated: the secret life of the synaptonemal complex at the centromeres PLoS Genet. 8(6), e1002807.

Thomas H. M., Harper J. A., Morgan W. G. 2001. Gross chromosome rearrangements are occurring in an accession of the grass Lolium rigidum. Chromosome Res. 9, 585–590.

Tilquin J. P. 1981. An unusual case of a complex heterozygote presenting no taxonomical problem in Chelidonium majus L. (Papaveraceae). Experientia 37, 341–342.

Tschermak-Woess E. 1947. Cytologische und embryologische untersuchungen an Rhoeo discolor.

Öesterr. Bot. Z. 94, 128–135.

Veltsos P., Keller I., Nichols R. A. 2009. Geographically localised bursts of ribosomal DNA mobility in the grasshopper Podisma pedestris. Heredity 103, 54–61.

Verma S. C., Ohri D. 1979. Breakdown of the classical meiotic system in Rhoeo spathacea (Commelinaceae). Cytologia 44, 91–102.

Verma RC, Vyas P, Raina SN. 1992. The effect of colchicine on meiosis in the Rhoeo discolor stabile complex translocation heterozygote. Genome 35, 611–613.

Walters M. S., Gerstel D. U. 1948. A cytological investigation of a tetraploid Rhoeo discolor. Am. J. Bot. 35, 141–150.

Wimber D. E. 1968. The nuclear cytology of bivalent and ring-forming rhoeos and their hybrids. Am. J. Bot. 55, 572–574.

Williams E., Heslop-Harrison J. 1975. Membrane-bounded cisternae associated with the chromosomes at first meiotic metaphase in microsporocytes of Rhoeo spathacea. Protoplasma 86, 285–289.

Youds J. L., Boulton S. J. 2011. The choice in meiosis – defining the factors that influence crossover or non-crossover formation. J. Cell Sci. 124, 501–513.

Zimmermann E. 1968. Mechanismus der nondisjunktionalen Chromosomenverteilung und die Ursachen der Pollensterilität bei Rhoeo spathacea. Chromosoma 25, 215–248.

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