Optimization of in vitro culture conditions influencing the initiation of raspberry (Rubus idaeus L. cv. Nawojka) cell suspension culture

Ewa Dziadczyk, Marcin Domaciuk, Piotr Dziadczyk, Iwona Pawelec, Ewa Szczuka, Józef Bednara

Abstract


The purpose of our investigation was to determine appropriate conditions for induction of raspberry (Rubus idaeus cv. Nawojka) cell suspension culture. The established callus culture obtained from leaf explants was used as an inoculum for cell culture initiation. Five combinations of plant growth regulators: 1) 4.0 mg l-1 IAA and 1.0 mg l-1 BAP; 2) 0.25 mg l-1 2,4-D; 3) 0.5 mg l-1 2,4-D; 4) 2.0 mg l-1 NAA and 2.0 mg l-1 BAP; 5) 4.0 mg l-1 NAA and 2.0 mg l-1 BAP, added into modified Murashige and Skoog (1962) medium, were tested in order to get the callus culture suitable for initiation of a cell suspension. The best callus (vigorously growing, healthy and friable) was obtained on the medium supplemented with 4.0 mg l-1 IAA and 1.0 mg l-1 BAP. To find the appropriate culture conditions for dispersing callus tissue in liquid medium into single cells and small aggregates, four combinations of plant hormones (auxins and cytokinins) were tested. The best culture medium for induction of raspberry cv. Nawojka cell suspension appeared to be the one supplemented with 1.0 mg l-1 2,4-D. Also the medium with 8.0 mg l-1 IAA and 1.0 mg l-1 BAP was similarly efficient.

Keywords


cell suspension culture; callus culture; raspberry; Rubus idaeus; plant growth regulators

Full Text:

PDF

References


Altman A. 2003. From plant tissue culture to biotechnology: scientific revolutions, abiotic stress tolerance, and forestry. In Vitro Cell. Dev. Biol.-Plant. 39: 75–84.

Anand R. P., Ganapathi A., Anbazhagan V. R., Vengadesan G., Selvaraj N. 2000. High frequency plant regeneration via somatic embryogenesis in cell suspension cultures of cowpea, Vigna unguiculata (L.) Walp. In Vitro Cell. Dev. Biol.-Plant. 36: 475–480.

Borejsza-Wysocki W., Hrazdin G. 1994. Establishment of callus and cell suspension cultures of raspberry (Rubus idaeus cv. Royalty). Plant Cell Tiss Organ Cult. 37: 213–216.

Brown J. T. 1990. The Initiation and Maintenance of Callus Cultures. In: Methods in Molecular Biology. Vol. 6, Plant Cell and Tissue Culture. J. W. Pollard, J. M. Walker (eds.). Humana Press, Clifton, New Jersey, 57–63.

Cortelazzo A. L., Marais M.-F., Joseleau J.-P. 1996. Changes in peroxidases in the suspension culture of Rubus fruticosus during growth. Plant Cell Tiss Organ Cult. 46: 27–33.

Debnath S. C. 2004. Clonal propagation of dwarf raspberry (Rubus pubescens Raf.) through in vitro axillary shoot proliferation. Plant Growth Regul. 43: 179–186.

Debnath S. C. 2007. A two-step procedure for in vitro multiplication of cloudberry (Rubus chamaemorus L.) shoots using bioreactor. Plant Cell Tiss Organ Cult. 88: 185–191.

Evans D. E., Coleman J. O. D., Kearns A. 2003. Plant Cell Culture. BIOS Scientific Publishers, 81–87.

Gamborg O. L. 2002. Plant tissue culture. Biotechnology. Milestones. In Vitro Cell. Dev. Biol.-Plant 38: 84–92.

Graham J., Woodhead M. 2009. Raspberries and Blackberries: The Genomics of Rubus. In: Genetics and Genomics of Rosaceae, Plant Genetics and Genomics: Crops and Models 6, DOI10.1007/978-0-387-77491-6 24, K. M. Folta, S. E. Gardiner (eds.), Springer Science+ Business Media, 507–524.

Joseleau J. P., Chambat G., Cortelazzo A. L., Faik A., Priem B., Ruel K. 1995. Oligosaccharides from xyloglucan affect the development of Rubus fruticosus cell suspension culture. Current Issues in Plant Molecular and Cellular Biology: 433–443.

Kanwar K., Kaushal B., Abrol S., Deepika R. 2008. Plant regeneration in Robinia pseudoacacia from cell suspension cultures. Biologia Plantarum 52 (1): 187–190.

Karam N. S., Jawad F. M., Arikat N. A., Shibli R. A. 2003. Growth and rosmarinic acid accumulation in callus, cell suspension, and root cultures of wild Salvia fruticosa. Plant Cell Tiss Organ Cult. 73: 117–121.

Llamoca-Z´arate R. M., Studart-Guimarães C., Landsmann J., Campos F. A. P. 1999. Establishment of callus and cell suspension cultures of Opuntia ficus-indica. Plant Cell Tiss Organ Cult. 58: 155–157.

Martinussen I., Nilsen G., Svenson L., Junttila O., Rapp K. 2004. In vitro propagation of cloudberry (Rubus chamaemorus). Plant Cell Tiss Organ Cult. 78: 43–49.

Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.

Salman M. N. 2002. Establishment of callus and cell suspension cultures from Gypsophila paniculata leaf segments and study of the attachment of host cells by Erwinia herbicola pv. Gypsophilae. Plant Cell Tiss Organ Cult. 69: 189–196.

Shekhawat M. S., Shekhawat N. S. 2011. Micropropagation of Arnebia hispidissima (Lehm).Dc. and production of alkannin from callus and cell suspension culture. Acta Physiol. Plant. 33: 1445–1450.

Sobczykiewicz D. 1992. Micropropagation of Raspberry (Rubus idaeus L.). In: Biotechnology in Agriculture and Forestry. Vol. 18. High Tech and Micripropagation II. Y. P. S. Bajaj (ed.), Springer-Verlag Berlin Heidelberg, 339–350.

Stella A., Braga M. R. 2002. Callus and cell suspension cultures of Rudgea jasminoides, a woody Rubiaceae tropical. Plant Cell Tiss Organ Cult. 68: 271–276.

Tsao C. W. V., Reed B. M. 2002. Gelling agents, silver nitrate, and sequestrene iron influence adventitious shoot and callus formation from Rubus leaves. In Vitro Cell. Dev. Biol.- Plant. 38: 29–32.

Vasil I. K. 2008. A history of plant biotechnology: from the Cell Theory of Schleiden and Schwann to biotech crops. Plant Cell Rep. 27: 1423–1440.

Vengadesan G., Ganapathi A., Anbazhagan V.R., Anand R.P. 2002. Somatic Embryogenesis in Cell Suspension Cultures of Acacia sinuata (LOUR.) MERR. In Vitro Cell. Dev. Biol.-Plant 38: 52–57.

Verpoorte R., Contin A., Memelink J. 2002. Biotechnology for the production of plant secondary metabolites. Phytochemistry Reviews 1: 13–25.

Vujovic T., Ruzic D., Cerovic R., Momirovic G. S. 2010. Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants. Plant Growth Regul. 61: 265–275.

Wu J. H., Miller S. A., Hall H. K., Mooney P. A. 2009. Factors affecting the effificiency of micropropagation from lateral buds and shoot tips of Rubus. Plant Cell Tiss. Organ. Cult. 99: 17–25.

Zheng D., Schröder G., Schröder J., Hrazdina G. 2001. Molecular and biochemical characterization of three aromatic polyketide synthase genes from Rubus idaeus. Plant Molecular Biology 46: 1–15.




DOI: http://dx.doi.org/10.17951/c.2013.68.2.15
Data publikacji: 2015-07-18 01:57:31
Data złożenia artykułu: 2015-07-18 00:47:06

Refbacks

  • There are currently no refbacks.


Copyright (c) 2015 Ewa Dziadczyk, Marcin Domaciuk, Piotr Dziadczyk, Iwona Pawelec, Ewa Szczuka, Józef Bednara

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.