Calcium oxalate crystals in the stem of Sida hermaphrodita (L.) Rusby (Malvaceae)

Agata Leszczuk, Ewa Szczuka, Kinga Stanisławek, Ilona Mazurkiewcz, Anna Kasprzyk


Observations of calcium oxalate crystals of the stem of an energetic plant S. hermaphrodita (L.) Rusby from the Malvaceae family, were performed using LM, DIC, and CLSM microscopes. The transversal and longitudinal sections showed the presence of calcium oxalate crystals in the parenchymal tissue distributed in various layers of the stem. The crystals occurred only in the form of druses. In the innermost part of the stem, i.e. in the pith, the calcium oxalate crystals occurred singly in individual cells. In the parenchyma cells separating sclerenchyma fibres and adjacent to the xylem, the crystals were observed individually in single cells, but the cells containing druses formed rows consisting of even several cells. The cortex contained the different-size druses scattered randomly within the cells. Druses differ in shape and size but they do not protrude beyond the cells although they very often fill them completely. The functions of calcium oxalate crystals are discussed.

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Borkowska H., Styk B. 1998. Ślazowiec pensylwański (Sida hermaphrodita Rusby). Uprawa i wykorzystanie. WAR, Lublin.

Brubaker C.L., Horner H.T. 1989. Development of epidermal crystals in leaflets of Stylosanthes guianensis (Leguminosae; Papilionoideae). Canadial Journal of Botany. 67: 1664–1670.

Faheed F., Mazen A., Abd Elmohsen S. 2013. Physiological and ultrastructural studies on calcium oxalate crystal formation in some plants. Turkish Journal of Botany. 37: 139–152.

Franceschi V. R., Horner H. T. Jr. 1980. Calcium Oxalate Crystals in Plants. The Botanical Review. 46 (4): 361–427.

Franceschi V. R., Nakata P. A. 2005. Calcium Oxalate in Plants: Formation and Function. Annual Review of Plant Biology. 56: 41–71.

Katayama H., Fujibayashi Y., Nagaoka S., Sugimura Y. 2007. Cell wall sheath surrounding calcium oxalate crystals in mulberry idioblasts. Protoplasma. 231: 245–248.

Kalembasa S., Wiśniewska B. 2006. Wpływ dawek azotu na plon biomasy ślazowca pensylwańskiego (Sida hermaphrodita Rusby) oraz zawartość w niej makroelementów. Acta Agrophysica. 8 (1): 127–138.

Kostman T.A., Franceschi V. R. 2000. Cell and calcium oxalate crystal growth is coordinated to achieve high-capacity calcium regulation in plants. Protoplasma. 214: 166–179.

Kuo-Huang L.L., Ku M.S.B., Franceschi V. R. 2007. Correlations between calcium oxalate crystals and photosynthetic activities in palisade cells of shade-adapted Peperomia glabella. Botanical Studies. 48: 155-164.

Meric C. 2009. Calcium Oxalate Crystals in Some Species of the Tribe Inuleae (Asteraceae). Acta Biologica Cracoviensia. 51 (1): 105–110.

Molano-Flores B. 2001. Herbivory and Calcium Concentrations Affect Calcium Oxalate Crystal Formation in Leaves of Sida (Malvaceae). Annals of Botany. 88: 387–391.

Nakata P. A. 2003. Advances in our understanding of calcium oxalate crystal formation and function in plants. Plant Science. 164: 901–909.

Pattar P.V., Jayaraj M. 2012. Pharmacognostic and phytochemical investigation of Sida cordifolia L.- A threatened medicinal herb. International Journal of Pharmacy and Pharmaceutical Sciences. 4(1): 114–117.

Volk G.M., Lynch-Holm V.J., Kostman T.A., Goss L.J., Franceschi V. R. 2002. The Role of Druse and Raphide Calcium Oxalate Crystals in Tissue Calcium Regulation in Pistia stratiotes Leaves. Plant Biology. 4: 34–45.

Webb M.A. 1999. Cell-Mediated Crystalization of Calcium Oxalate in Plants. The Plant Cell. 11: 751–761

Zindler-Frank E., Hönow R., Hesse A. 2001. Calcium and oxalate content of the leaves of Phaseolus vulgaris at different calcium supply in relation to calcium oxalate crystal formation. Journal of Plant Physiology. 158: 139–144.

Data publikacji: 2015-05-23 17:51:53
Data złożenia artykułu: 2015-05-09 15:58:00


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